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KMID : 1007520090180040996
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Food Science and Biotechnology
2009 Volume.18 No. 4 p.996 ~ p.1000
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Optimization of Extraction Conditions for Total Phenolics from Sapium japonicum Using a Pressurized Liquid Extractor
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Kim Mi-Bo
Park Jae-Sung
Lim Sang-Bin
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Abstract
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Sapium japonicum was extracted by a pressurized liquid. Operating parameters such as the type and the ratio ofsolvent to water, temperature, pressure, and number of extractions were investigated as the main variables that influence theextraction efficiencies of total phenolics (TP). MeOH extracted the highest level of TP as 50.4 mg GAE/g compared to 48.8and 27.2 mg GAE/g with H2O and EtOH, respectively. EtOH:H2O (40:60, v/v) was found to be the best solvent for TPextraction as 90.3 mg GAE/g compared to 85.0 and 84.3 mg GAE/g in 40:60 and 60:40 of MeOH:H2O, respectively. TP wereincreased with the increase of the number of extraction steps. TP content was increased by 11% as the extraction temperaturewas increased from 40 (97.4) to 50oC (108.3 mg GAE/g). The optimum extraction conditions of TP were; extraction solvent,EtOH:H2O (40:60, v/v); temperature, 50oC; pressure, 10.2 MPa; 2 extraction steps.
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KEYWORD
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total phenolic, total soluble solid, pressurized liquid extraction, Sapium japonicum
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